基因叔叔：科普、期刊導讀(Uncle Gene)

不但給你最強基因科普，還免費送你期刊導讀；救人一命勝造七級浮屠，想知道就在基因叔叔。真是好詩，好詩！ EGFR,KRAS,NRAS,HRAS,TET2,JAK2,P53,DNMT3A,PIK3CA,MED12,BRAF,PI3K,KIT,FLT3,ASXL1,BRCA-1,BRCA-2,ABL,AKT3,SNP,breast cancer,colorectal cancer,gastric cancer,Liver cancer,Lung cancer,Leukemia cancer,Ovary cancer,Prostate cancer,femtopath,genonfire,oncogene,prevention,drugs,information for genes,OIS,screening,journal, Association between Tumor Epidermal Growth Factor Receptor Mutation and Pulmonary Tuberculosis in Patients with Adenocarcinoma of the Lungs Epidermal growth factor receptor (EGFR) in lung cancer: an overview and update Targeting the EGFR Pathway for Cancer Therapy EGFR mutation testing in lung cancer: a review of available methods and their use for analysis of tumour tissue and cytology samples Epidermal Growth Factor Receptor in Pancreatic Cancer Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer PIK3CA Mutations Frequently Coexist with EGFR/KRAS Mutations in Non-Small Cell Lung Cancer and Suggest Poor Prognosis in EGFR/KRAS Wildtype Subgroup Lei Wang1,2., Haichuan Hu1,2., Yunjian Pan1,2., Rui Wang1,2, Yuan Li2,3, Lei Shen2,3, Yongfu Yu4, Hang Li1,2, Deng Cai1,2, Yihua Sun1,2, Haiquan Chen1,2 1Department of Thoracic Surgery, Shanghai Medical College, Fudan University Shanghai Cancer Center, Shanghai, China, 2Department of Oncology, Shanghai Medical College, Fudan University Shanghai Cancer Center, Shanghai, China, 3Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China, Immunohistochemistry Immunohistochemistry Immunohistochemistry Immunohistochemistry Immunohistochemistry (IHC) involves the process of selectively imaging antigens (e.g. proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.[1] IHC takes its name from the roots "immuno", in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). Albert Coons conceptualized and first implemented the procedure in 1941.[2] Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis).[3] Immunohistochemistry is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. Visualising an antibody-antigen interaction can be accomplished in a number of ways. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing reaction (see immunoperoxidase staining).[4] Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein or rhodamine (see immunofluorescence). Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids strands including DNA, cDNA or RNA. The key difference between dPCR and traditional PCR lies in the method of measuring nucleic acids amounts, with the former being a more precise method than PCR, though also more prone to error in the hands of inexperienced users.[1]:217 A “digital” measurement quantitatively and discretely measures a certain variable, whereas an “analog” measurement extrapolates certain measurements based on measured patterns. PCR carries out one reaction per single sample. dPCR also carries out a single reaction within a sample, however the sample is separated into a large number of partitions and the reaction is carried out in each partition individually. This separation allows a more reliable collection and sensitive measurement of nucleic acid amounts. The method has been demonstrated as useful for studying variations in gene sequences — such as copy number variants and point mutations — and it is routinely used for clonal[disambiguation needed] amplification of samples for next-generation sequencing. ihc ihca ihc protocol ihc原理 ihc4 ihc 染色 ihc染色原理 ihc 步驟 ihc staining ihc-p ihc protocol ihc stain ihc 染色 ihc 步驟 ihc staining ihc 中文 ihc world ihc score ihc twc ihc blocking ddpcr ddpcr原理 ddpcr supermix ddpcr bio rad ddpcr protocol ddpcr ngs ddpcr multiplex ddpcr ppt ddpcr supermix for probes (no dutp) ddpcr hiv ddpcr 原理 ddpcr supermix ddpcr bio rad ddpcr protocol ddpcr ngs ddpcr multiplex ddpcr ppt ddpcr supermix for probes (no dutp) ddpcr hiv ddpcr multiplexing ngs ngsff ng sdh ngstyle ngse ngsl ngs 初始化錯誤 ngsp ngspice ng switch ngs 原理 ngs 初始化錯誤 ngs hacking detected ngs illumina ngs airport ngs data analysis ngs data ngs wiki ngs 分析 ngs 應用 general genesis generic general 中文 generation geneva generous 中文 generator generous generate gene 中文 gene ontology gene therapy gene kelly gene simmons gene expression gene hackman gene card gene expression 中文 gene wilder krasnodar kras krasnoyarsk kras mutation kras巧克力 krasnoyarsk 中文 krashen kraso kras基因 krasny krym kras mutation kras 巧克力 kras 基因 kras wild type kras g12d kras mutation中文 kras nras kras lung cancer kras chocolate kras pathway egfr egfr calculator egfr計算 egfr mutation egfr 中文 egfr公式 egfr是什麼 egfr過低 egfr檢驗值 gfr正常值 egfr calculator egfr mutation egfr 中文 egfr 公式 egfr 計算 egfr tki egfr 肺癌 egfr inhibitor egfr cancer gfr 正常值 p53 p53 pathway p530 p53 apoptosis p53 p21 p53 mdm2 p53 mutation p53 antibody p53 cancer p53 protein p53 gene p53 pathway p53 apoptosis p53 p21 p53 mdm2 p53 mutation p53 antibody p53 cancer p53 protein p53 cell cycle 抗體抗體抗原抗體英文抗體結構抗體是什麼抗體藥物抗體力價抗體成分抗體製造抗體篩檢抗體抗原抗體英文抗體成分抗體蛋白質抗體 fc 抗體結構抗體受體抗體日文抗體輕鏈抗體陽性 santa cruz santa cruz 景點 santa cruz bike santa cruz 滑板 santa cruz 台灣 santa cruz nomad santa cruz bolivia santa cruz beach boardwalk santa cruz weather santa cruz skateboard santa cruz 景點 santa cruz biotechnology santa cruz bike santa cruz 滑板 santa cruz 台灣 santa cruz nomad santa cruz beach boardwalk santa cruz bolivia santa cruz weather santa cruz de tenerife cancer cancer 中文 cancer fund cancer research cancer cell cancer 星座 cancer council cancer research impact factor cancer ptt cancer council 防曬 cancer 中文 cancer fund cancer research cancer cell cancer 星座 cancer council cancer research impact factor cancer ptt cancer council 防曬 cancer letters 期刊期刊英文期刊縮寫期刊排名期刊下載期刊查詢期刊排名查詢期刊點數期刊格式期刊投稿期刊英文期刊點數期刊格式期刊論文期刊縮寫期刊 impact factor 期刊查詢期刊下載期刊排名期刊論文差別

部落格全站分類：醫療保健

11月 27 週二 201816:28
期刊導讀：母乳對一歲嬰兒的腸道菌相發展具有關鍵調節影響

腸道菌相-1歲嬰兒-封面.tif

於先前文章「什麼是腸道菌相(human gut microbiota)？」提及，新生兒從一離開母體接觸到外界，就開始培養屬於自己的腸道菌，其中有99%就坐落於腸道之中，其中影響菌相組成的因素包含：母體的健康及營養、分娩方式、有無使用抗生素、新生兒喝母乳或配方奶，以及何時開始斷奶並食用固體食物。

(繼續閱讀...)

基因叔叔發表在痞客邦留言(0) 人氣(777)

個人分類：基因叔叔講講醫療

9月 26 週三 201817:44
期刊導讀：與免疫檢查點抑製劑 (Immune Checkpoint Inhibitor) 相關的致命毒性作用

Immune checkpoint inhibitor-封面.tif

免疫檢查點抑制劑 (Immune checkpoint inhibitors，簡稱ICIs) 是腫瘤免疫治療的希望。與化療藥物不同，這些新型藥物對腫瘤細胞並不具有直接的細胞毒性，而是通過阻斷腫瘤對免疫系統的抑制、從而增強機體的內源性抗腫瘤免疫反應。ICIs是一種有效的新型抗癌藥物，已顯著延長了幾種難治癌症的生存，尤其黑色素瘤和非小細胞肺癌；此外，ICIs也納入多種實體腫瘤和血液惡性腫瘤的治療指南。

(繼續閱讀...)

基因叔叔發表在痞客邦留言(1) 人氣(6,245)

個人分類：基因叔叔講講醫療

7月 03 週二 201815:22
期刊導讀：人類腸道微生物的腸型分析

圖片1.jpg

Enterotypes of the human gut microbiome
Nature. 2011 May 12;473(7346):174-80.

(繼續閱讀...)

基因叔叔發表在痞客邦留言(0) 人氣(1,096)

個人分類：基因叔叔講講醫療

6月 22 週五 201813:46
期刊導讀：腸道微生物體 (Gut microbiome) 對健康成人的影響

Microbiota-封面.tif

腸道菌叢(Gut Microbiota)主要指棲息在人體腸道內的龐大數目之細菌族群。正常情形下，預估超過 10¹⁴的菌群居住於腸胃道，因此其對於人體的各種生理及病理現象影響相當明顯且重要。

(繼續閱讀...)

基因叔叔發表在痞客邦留言(0) 人氣(7,157)

個人分類：基因叔叔講講醫療

6月 19 週二 201811:22
期刊導讀：人體微生物對健康與疾病的關係

圖片2.jpg

The human microbiome: at the interface of health and disease
Nat Rev Genet. 2012 Mar 13;13(4):260-70.

(繼續閱讀...)

基因叔叔發表在痞客邦留言(0) 人氣(1,420)

個人分類：基因叔叔講講醫療

6月 05 週二 201818:58
期刊導讀：探討印度傳統辣木 (Moringa oleifera Lam.) 萃取物對腸道微生物環境的影響

辣木腸道-封面.tif

先前文章提及，腸道猶如人體的第二個大腦，影響著一個人的身體。因此近期許多科學家針對腸道進行研究，窺探裡頭究竟有什麼？到目前的研究指出，腸道微生物似乎就是主宰著這一切的源頭。腸道微生物群在腸道內處於一個動態平衡的狀態，一旦這動態平衡受到破壞，就很有可能引起疾病。對此，異想天開的科學家們，就會想探討哪些因素會影響著腸道微生物，例如：飲食、運動…等等。

(繼續閱讀...)

基因叔叔發表在痞客邦留言(0) 人氣(1,441)

個人分類：基因叔叔講講醫療

6月 05 週二 201812:29
富含多酚與咖啡因的後發酵普洱茶能透過重整小鼠腸道平衡來改善因飲食而產生的代謝症候群

圖片1.jpg

Polyphenol- and caffeine-rich post-fermented Pu-er tea improves diet-induced metabolic syndrome by remodeling intestinal homeostasis in mice
Infect Immun. 2017 Dec 19;86(1).

(繼續閱讀...)

基因叔叔發表在痞客邦留言(0) 人氣(303)

個人分類：基因叔叔講講醫療

4月 24 週二 201815:51
期刊導讀：腸道菌相對癌症、免疫及癌症免疫治療的影響

腸道菌相與免疫-封面.tif

由上篇文章【腸道菌相】已知，腸道菌相對於人體的健康影響很大，近年越來越多的研究證實腸道微生物組 (gut microbiome) 會影響癌症治療的反應，特別是阻斷免疫檢查點 (Immune checkpoint) 的治療方式。腸道菌相為什麼會影響？又是怎麼影響的？這就要先了解癌症相關的免疫監視 (immunosurveillance) 以及影響宿主和抗腫瘤免疫 (包含腸道菌相) 等相關因素，這些因素對於免疫治療的反應皆會互相影響。

(繼續閱讀...)

基因叔叔發表在痞客邦留言(4) 人氣(653)

個人分類：基因叔叔講講醫療

4月 03 週二 201820:33
期刊導讀：糞便DNA v.s. 糞便免疫化學測試

FITvsStoolDNA-封面.tif

許多隨機對照試驗證實以愈創木脂測試 (guaiac-based fecal occult blood tests, 簡稱 gFOBTs) 可以有效減少大腸直腸癌 (Colorectal cancer, CRC) 的死亡率；然而，傳統的gFOBTs偵測CRC及癌前病變的敏感度較低。後來以糞便免疫化學測試 (Fecal immunochemical tests, 簡稱FITs) 來檢測隱藏在糞便中無法以肉眼看見的血液，其診斷結果相較於gFOBTs較好。但是近期有篇文獻指出FITs對於CRC有較差的敏感度 (79%) 及特異性 (94%)。

(繼續閱讀...)

基因叔叔發表在痞客邦留言(0) 人氣(797)

個人分類：基因叔叔講講醫療

2月 08 週四 201812:42
期刊導讀：間質幹細胞在惡性血液性腫瘤中之作用及應用

MSC and myeloid malignancied-封面.tif

(繼續閱讀...)

基因叔叔發表在痞客邦留言(0) 人氣(929)

個人分類：基因叔叔講講醫療