基因叔叔：科普、期刊導讀(Uncle Gene)

不但給你最強基因科普，還免費送你期刊導讀；救人一命勝造七級浮屠，想知道就在基因叔叔。真是好詩，好詩！ EGFR,KRAS,NRAS,HRAS,TET2,JAK2,P53,DNMT3A,PIK3CA,MED12,BRAF,PI3K,KIT,FLT3,ASXL1,BRCA-1,BRCA-2,ABL,AKT3,SNP,breast cancer,colorectal cancer,gastric cancer,Liver cancer,Lung cancer,Leukemia cancer,Ovary cancer,Prostate cancer,femtopath,genonfire,oncogene,prevention,drugs,information for genes,OIS,screening,journal, Association between Tumor Epidermal Growth Factor Receptor Mutation and Pulmonary Tuberculosis in Patients with Adenocarcinoma of the Lungs Epidermal growth factor receptor (EGFR) in lung cancer: an overview and update Targeting the EGFR Pathway for Cancer Therapy EGFR mutation testing in lung cancer: a review of available methods and their use for analysis of tumour tissue and cytology samples Epidermal Growth Factor Receptor in Pancreatic Cancer Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer PIK3CA Mutations Frequently Coexist with EGFR/KRAS Mutations in Non-Small Cell Lung Cancer and Suggest Poor Prognosis in EGFR/KRAS Wildtype Subgroup Lei Wang1,2., Haichuan Hu1,2., Yunjian Pan1,2., Rui Wang1,2, Yuan Li2,3, Lei Shen2,3, Yongfu Yu4, Hang Li1,2, Deng Cai1,2, Yihua Sun1,2, Haiquan Chen1,2 1Department of Thoracic Surgery, Shanghai Medical College, Fudan University Shanghai Cancer Center, Shanghai, China, 2Department of Oncology, Shanghai Medical College, Fudan University Shanghai Cancer Center, Shanghai, China, 3Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China, Immunohistochemistry Immunohistochemistry Immunohistochemistry Immunohistochemistry Immunohistochemistry (IHC) involves the process of selectively imaging antigens (e.g. proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.[1] IHC takes its name from the roots "immuno", in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). Albert Coons conceptualized and first implemented the procedure in 1941.[2] Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis).[3] Immunohistochemistry is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. Visualising an antibody-antigen interaction can be accomplished in a number of ways. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing reaction (see immunoperoxidase staining).[4] Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein or rhodamine (see immunofluorescence). Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids strands including DNA, cDNA or RNA. The key difference between dPCR and traditional PCR lies in the method of measuring nucleic acids amounts, with the former being a more precise method than PCR, though also more prone to error in the hands of inexperienced users.[1]:217 A “digital” measurement quantitatively and discretely measures a certain variable, whereas an “analog” measurement extrapolates certain measurements based on measured patterns. PCR carries out one reaction per single sample. dPCR also carries out a single reaction within a sample, however the sample is separated into a large number of partitions and the reaction is carried out in each partition individually. This separation allows a more reliable collection and sensitive measurement of nucleic acid amounts. The method has been demonstrated as useful for studying variations in gene sequences — such as copy number variants and point mutations — and it is routinely used for clonal[disambiguation needed] amplification of samples for next-generation sequencing. ihc ihca ihc protocol ihc原理 ihc4 ihc 染色 ihc染色原理 ihc 步驟 ihc staining ihc-p ihc protocol ihc stain ihc 染色 ihc 步驟 ihc staining ihc 中文 ihc world ihc score ihc twc ihc blocking ddpcr ddpcr原理 ddpcr supermix ddpcr bio rad ddpcr protocol ddpcr ngs ddpcr multiplex ddpcr ppt ddpcr supermix for probes (no dutp) ddpcr hiv ddpcr 原理 ddpcr supermix ddpcr bio rad ddpcr protocol ddpcr ngs ddpcr multiplex ddpcr ppt ddpcr supermix for probes (no dutp) ddpcr hiv ddpcr multiplexing ngs ngsff ng sdh ngstyle ngse ngsl ngs 初始化錯誤 ngsp ngspice ng switch ngs 原理 ngs 初始化錯誤 ngs hacking detected ngs illumina ngs airport ngs data analysis ngs data ngs wiki ngs 分析 ngs 應用 general genesis generic general 中文 generation geneva generous 中文 generator generous generate gene 中文 gene ontology gene therapy gene kelly gene simmons gene expression gene hackman gene card gene expression 中文 gene wilder krasnodar kras krasnoyarsk kras mutation kras巧克力 krasnoyarsk 中文 krashen kraso kras基因 krasny krym kras mutation kras 巧克力 kras 基因 kras wild type kras g12d kras mutation中文 kras nras kras lung cancer kras chocolate kras pathway egfr egfr calculator egfr計算 egfr mutation egfr 中文 egfr公式 egfr是什麼 egfr過低 egfr檢驗值 gfr正常值 egfr calculator egfr mutation egfr 中文 egfr 公式 egfr 計算 egfr tki egfr 肺癌 egfr inhibitor egfr cancer gfr 正常值 p53 p53 pathway p530 p53 apoptosis p53 p21 p53 mdm2 p53 mutation p53 antibody p53 cancer p53 protein p53 gene p53 pathway p53 apoptosis p53 p21 p53 mdm2 p53 mutation p53 antibody p53 cancer p53 protein p53 cell cycle 抗體抗體抗原抗體英文抗體結構抗體是什麼抗體藥物抗體力價抗體成分抗體製造抗體篩檢抗體抗原抗體英文抗體成分抗體蛋白質抗體 fc 抗體結構抗體受體抗體日文抗體輕鏈抗體陽性 santa cruz santa cruz 景點 santa cruz bike santa cruz 滑板 santa cruz 台灣 santa cruz nomad santa cruz bolivia santa cruz beach boardwalk santa cruz weather santa cruz skateboard santa cruz 景點 santa cruz biotechnology santa cruz bike santa cruz 滑板 santa cruz 台灣 santa cruz nomad santa cruz beach boardwalk santa cruz bolivia santa cruz weather santa cruz de tenerife cancer cancer 中文 cancer fund cancer research cancer cell cancer 星座 cancer council cancer research impact factor cancer ptt cancer council 防曬 cancer 中文 cancer fund cancer research cancer cell cancer 星座 cancer council cancer research impact factor cancer ptt cancer council 防曬 cancer letters 期刊期刊英文期刊縮寫期刊排名期刊下載期刊查詢期刊排名查詢期刊點數期刊格式期刊投稿期刊英文期刊點數期刊格式期刊論文期刊縮寫期刊 impact factor 期刊查詢期刊下載期刊排名期刊論文差別

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Mar 03 Fri 2017 22:31
DNMT3A是惡性白血病的小三還是正宮？

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從上篇文章【惡性白血病的幕後主使者】已經簡單得知DNMT3A是誰了，接下來要告訴大家，DNMT3A在不同血液惡性腫瘤中突變的頻率。首先由下圖可以看到不同的血液惡性腫瘤可能是DNMT3A突變外加其他基因再次突變所導致 (圖一)

DNMT3A-other mutation.tif

圖一、血液惡性腫瘤之相關突變基因

接著，DNMT3A在不同血液惡性腫瘤的病人身上，突變位置隨疾病類型不同而不同，下圖是DNMT3A突變位置整以及ALL、MDS及T-ALL/Lymphomas各突變位置所占比例 (如圖二)

DNMT3A mutation site.tif

圖二、DNMT3A突變位點

再者，DNMT3A在不同類型血液惡性腫瘤中的突變頻率 (如圖三)

DNMT3A mutation rate.tif

圖三、不同血液惡性腫瘤之DNMT3A突變頻率

從上圖可以觀察到，DNMT3A在AML類別中，突變的頻率最高！

最後，根據TCGA (The Cancer Genome Atlas)的資料庫統計，AML除了DNMT3A基因突變之外，還有NPM1、FLT3及IDH1基因產生突變或者是基因缺失，下圖為各基因突變率及各疾病相關的突變基因。(如圖四)

DNMT3A-TCGA.tif

圖四、不同血液惡性腫瘤中相關突變基因

Golden叔叔

參考文獻

1.Liubin Yang et.al, DNMT3A in haematological malignancies., NATURE REVIEWS, 2015, VOLUME 15

DNMT3A AML MDS MPN PTCL T-ALL 急性骨髓性血癌骨髓化生不良症候群骨隨增殖性腫瘤惡性淋巴癌急性淋巴球血癌

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